质粒名称: | pLM-AMA18.0 dCas9-VPR |
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出品公司: | Addgene |
目录编号: | 138945 |
存储实验室: | Arnold Driessen |
载体骨架基本信息 |
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载体名称: | pDSM-JAK-109 |
载体出品公司: | DSM |
空载体大小: | 10170 bp |
完整质粒大小: | -- |
载体修饰: | pGpda:DsRed SKL已删除 |
载体类型: | CRISPR,合成生物学;AMA1自主复制真菌质粒 |
筛选标记: | 真菌选择:特比萘芬(ergA)/静脉霉素/博莱霉素(ble) |
载体抗性: | 氯霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers |
物种来源: | 合成的真菌表达,红色青霉 |
插入大小: | 21950 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子: | p40S(AN0465)巢状曲霉 |
标签/融合蛋白: | NLS |
克隆方法: | Gibson克隆 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pLM-AMA18.0 dCas9-VPR was a gift from Arnold Driessen (Addgene plasmid # 138945 ; http://n2t.net/addgene:138945 ; RRID:Addgene_138945) |
参考文献部分: | CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. 10.1038/s41598-020-80864-3 PubMed 33441979 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLM-AMA18.0 dCas9-VPR 21949 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers. ACCESSION . VERSION . KEYWORDS pLM-AMA18.0 dCas9-VPR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 21949) AUTHORS Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM TITLE CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. JOURNAL Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. PUBMED 33441979 REFERENCE 2 (bases 1 to 21949) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..21949 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1415..5518 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9m4 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D10A, D839A, H840A, and N863A (Mali et al., 2013)" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5531..5551 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5576..5725 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 5750..5770 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6200..6556 /codon_start=1 /product="transcriptional activation domain of human RelA, also known as p65 (O'Shea and Perkins, 2008)" /label=RelA (p65) AD /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD EDFSSIADMDFSALL" CDS 6575..7144 /codon_start=1 /product="transcriptional activation domain from the human herpesvirus 4 (Epstein-Barr virus) replication and transcription activator Rta/BRLF1 (Hardwick et al., 1992; Chavez et al., 2015)" /label=Rta AD /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL DTFLNDECLLHAMHISTGLSIFDTSLF" intron 8316..8431 /gene="gpdA" /label=gpdA intron /note="intron from the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al., 1990)" misc_RNA complement(10607..10682) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(10776..11099) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS DAA" primer_bind 10781..10800 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 11009..11025 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 11026..11082 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(11095..11111) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 11119..11135 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11143..11173) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11188..11209 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." intron 11281..11396 /gene="gpdA" /label=gpdA intron /note="intron from the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al., 1990)" primer_bind complement(11918..11937) /label=T7 /note="T7 promoter, forward primer" promoter complement(11919..11937) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(11947..11963) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 12105..12560 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(12192..12211) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12402..12423 /label=F1ori-F /note="F1 origin, forward primer" promoter 12586..12690 /gene="bla" /label=AmpR promoter promoter 13010..13112 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 13113..13772 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(13179..13198) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 14161..14749 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 14650..14669 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 14903..14920 /label=L4440 /note="L4440 vector, forward primer" protein_bind 15037..15058 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 15073..15103 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 15111..15127 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 15135..15151 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 15170..15190 /label=T3 /note="T3 promoter, forward primer" promoter 15172..15190 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" CDS 15982..16356 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" terminator 16441..16688 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(16457..16475) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" rep_origin 16698..21946 /label=AMA1 /note="replication origin from Aspergillus nidulans (Aleksenko and Clutterbuck, 1996)" ORIGIN 1 gaattcctgc agccccagat catcctgtct tcagtcttaa cgctgcaaga attcaagctt 61 ggaggagtta tagacggtcc ggcataggta aggagagatt agatatttcg acttcccgat 121 tccttagtct ccttagtctg gaaactgaga aaggcctgaa ctcgttcaat ctattagctc 181 taatcacgta gtctcaacta gaatcctgaa caggcgccgg aaccagtcca aatgcgtgga 241 gtacagcaag tccagtcaaa tcacgattag aatggccata tataagcaaa aaacccttgg 301 acaatacgtc cgatcgacac caaagcaatc tggtacatca cccttcttat atacagggta 361 gccggaattg tattgactct atacgaagcc gcaggtttac cagcacagag gtgcgatagc 421 tacacgaatg tagagtgaag gcattacaac cgattgctaa gtgtcataga ggctaagttt 481 cacggctcaa ggagtctgtg gaggatacct gatataagaa ttgagagtta tactccggag 541 gttatgttag tacctcaaaa atgtgaagta caacgaaact cgaacttcga atgacaatag 601 cttggctggc tgttagtcaa ctgaggtaca acgtcatctt gcaattagtt atgttctttg 661 ctagatagtg agctgctgta aacttgtagt tcttagaaac atgtctggag tctagagtcg 721 agacttgtga ttggtgtaaa ttagtggggg tccggagcgg aataacaatc gaggtcagcc 781 tcctgggtct gtcagtaagc taaccacgaa aggacaagcg tagacgaggt cacggggtac 841 tggcctagcc ggcttcggct agcgcccacg aaatagggca acgtcgaagc gagagacaca 901 cacggattcc acgatcgccg acgacaagca ctcaactcca cgacaaccca cgacaaccct 961 tgccgctgcg ttcttggtga agggctgcag gattagaggt gagtatttta ttttctggct 1021 attgtgatct ttttcccccc cggtttccgc tggttctatc ttgtcgagcg agtcggtgcg 1081 ccttcgatcg gtctgtcaaa tttccatccg tccactcgtt cacccggtat cgatacaaga 1141 gggaatggag cattacgaca ggggagcaga ggcagaactc tcgatgttgc tacttttttt 1201 gcttggaaca acttccttga aagattcttt tctgaatgcg gctgggaaac tgcgaattgg 1261 atatccagcg aaaggttacc gacagactca cggccatcga taccactcag cgggatagaa 1321 ttgaaggcat tcggacatta gatgatcagc ttcgcttgtt tcgactcgtg ctaatcaatt 1381 atccctggac agtcccccac atagacagca aacaatggac aagaagtatt ctatcggact 1441 ggccatcggg actaatagcg tcgggtgggc cgtgatcact gacgagtaca aggtgccctc 1501 taagaagttc aaggtgctcg ggaacaccga ccggcattcc atcaagaaaa atctgatcgg 1561 agctctcctc tttgattcag gggaaaccgc tgaagcaacc cgcctcaagc ggactgctag 1621 acggcggtac accaggagga agaaccggat ttgttacctt caagagatat tctccaacga 1681 aatggcaaag gtcgacgaca gcttcttcca taggctggaa gaatcattcc tcgtggaaga 1741 ggataagaag catgaacggc atcccatctt cggtaatatc gtcgacgagg tggcctatca 1801 cgagaaatac ccaaccatct accatcttcg caaaaagctg gtggactcaa ccgacaaggc 1861 agacctccgg cttatctacc tggccctggc ccacatgatc aagttcagag gccacttcct 1921 gatcgagggc gacctcaatc ctgacaatag cgatgtggat aaactgttca tccagctggt 1981 gcagacttac aaccagctct ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa 2041 ggccattctg tcagcccggc tgtcaaagag ccgcagactt gagaatctta tcgctcagct 2101 gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt gctctttcac ttgggctgac 2161 tcccaatttc aagtctaatt tcgacctggc agaggatgcc aagctgcaac tgtccaagga 2221 cacctatgat gacgatctcg acaacctcct ggcccagatc ggtgaccaat acgccgacct 2281 tttccttgct gctaagaatc tttctgacgc catcctgctg tctgacattc tccgcgtgaa 2341 cactgaaatc accaaggccc ctctttcagc ttcaatgatt aagcggtatg atgagcacca 2401 ccaggacctg accctgctta aggcactcgt ccggcagcag cttccggaga agtacaagga 2461 aatcttcttt gaccagtcaa agaatggata cgccggctac atcgacggag gtgcctccca 2521 agaggaattt tataagttta tcaaacctat ccttgagaag atggacggca ccgaagagct 2581 cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag cgcactttcg acaatgggag 2641 cattccccac cagatccatc ttggggagct tcacgccatc cttcggcgcc aagaggactt 2701 ctaccccttt cttaaggaca acagggagaa gattgagaaa attctcactt tccgcatccc 2761 ctactacgtg ggacccctcg ccagaggaaa tagccggttt gcttggatga ccagaaagtc 2821 agaagaaact atcactccct ggaacttcga agaggtggtg gacaagggag ccagcgctca 2881 gtcattcatc gaacggatga ctaacttcga taagaacctc cccaatgaga aggtcctgcc 2941 gaaacattcc ctgctctacg agtactttac cgtgtacaac gagctgacca aggtgaaata 3001 tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt 3061 ggaccttctg ttcaagacca atagaaaggt gaccgtgaag cagctgaagg aggactattt 3121 caagaaaatt gaatgcttcg actctgtgga gattagcggg gtcgaagatc ggttcaacgc 3181 aagcctgggt acttaccatg atctgcttaa gatcatcaag gacaaggatt ttctggacaa 3241 tgaggagaac gaggacatcc ttgaggacat tgtcctgact ctcactctgt tcgaggaccg 3301 ggaaatgatc gaggagaggc ttaagaccta cgcccatctg ttcgacgata aagtgatgaa 3361 gcaacttaaa cggagaagat ataccggatg gggacgcctt agccgcaaac tcatcaacgg 3421 aatccgggac aaacagagcg gaaagaccat tcttgatttc cttaagagcg acggattcgc 3481 taatcgcaac ttcatgcaac ttatccatga tgattccctg acctttaagg aggacatcca 3541 gaaggcccaa gtgtctggac aaggtgactc actgcacgag catatcgcaa atctggctgg 3601 ttcacccgct attaagaagg gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa 3661 ggtgatgggt cgccataaac cagagaacat tgtcatcgag atggccaggg aaaaccagac 3721 tacccagaag ggacagaaga acagcaggga gcggatgaaa agaattgagg aagggattaa 3781 ggagctcggg tcacagatcc ttaaagagca cccggtggaa aacacccagc ttcagaatga 3841 gaagctctat ctgtactacc ttcaaaatgg acgcgatatg tatgtggacc aagagcttga 3901 tatcaacagg ctctcagact acgacgtggc tgccatcgtc cctcagagct tcctcaaaga 3961 cgactcaatt gacaataagg tgctgactcg ctcagacaag gcccggggaa agtcagataa 4021 cgtgccctca gaggaagtcg tgaaaaagat gaagaactat tggcgccagc ttctgaacgc 4081 aaagctgatc actcagcgga agttcgacaa tctcactaag gctgagaggg gcggactgag 4141 cgaactggac aaagcaggat tcattaaacg gcaacttgtg gagactcggc agattactaa 4201 acatgtcgcc caaatccttg actcacgcat gaataccaag tacgacgaaa acgacaaact 4261 tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga 4321 ctttcaattc tacaaagtgc gggagatcaa taactatcat catgctcatg acgcatatct 4381 gaatgccgtg gtgggaaccg ccctgatcaa gaagtaccca aagctggaaa gcgagttcgt 4441 gtacggagac tacaaggtct acgacgtgcg caagatgatt gccaaatctg agcaggagat 4501 cggaaaggcc accgcaaagt acttcttcta cagcaacatc atgaatttct tcaagaccga 4561 aatcaccctt gcaaacggtg agatccggaa gaggccgctc atcgagacta atggggagac 4621 tggcgaaatc gtgtgggaca agggcagaga tttcgctacc gtgcgcaaag tgctttctat 4681 gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc 4741 aatcctcccc aagcgcaact ccgacaagct cattgcaagg aagaaggatt gggaccctaa 4801 gaagtacggc ggattcgatt caccaactgt ggcttattct gtcctggtcg tggctaaggt 4861 ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa ctgctgggta tcaccattat 4921 ggagcgcagc tccttcgaga agaacccaat tgactttctc gaagccaaag gttacaagga 4981 agtcaagaag gaccttatca tcaagctccc aaagtatagc ctgttcgaac tggagaatgg 5041 gcggaagcgg atgctcgcct ccgctggcga acttcagaag ggtaatgagc tggctctccc 5101 ctccaagtac gtgaatttcc tctaccttgc aagccattac gagaagctga aggggagccc 5161 cgaggacaac gagcaaaagc aactgtttgt ggagcagcat aagcattatc tggacgagat 5221 cattgagcag atttccgagt tttctaaacg cgtcattctc gctgatgcca acctcgataa 5281 agtccttagc gcatacaata agcacagaga caaaccaatt cgggagcagg ctgagaatat 5341 catccacctg ttcaccctca ccaatcttgg tgcccctgcc gcattcaagt acttcgacac 5401 caccatcgac cggaaacgct atacctccac caaagaagtg ctggacgcca ccctcatcca 5461 ccagagcatc accggacttt acgaaactcg gattgacctc tcacagctcg gaggggatag 5521 cagggctgac cccaagaaga agaggaaggt ggaggccagc ggttccggac gggctgacgc 5581 attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga 5641 catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct 5701 tgatgatttc gacctggaca tgctgattaa ctctagaagt tccggatctc cgaaaaagaa 5761 acgcaaagtt ggtagccagt acctgcccga caccgacgac cggcaccgga tcgaggaaaa 5821 gcggaagcgg acctacgaga cattcaagag catcatgaag aagtccccct tcagcggccc 5881 caccgaccct agacctccac ctagaagaat cgccgtgccc agcagatcca gcgccagcgt 5941 gccaaaacct gccccccagc cttacccctt caccagcagc ctgagcacca tcaactacga 6001 cgagttccct accatggtgt tccccagcgg ccagatctct caggcctctg ctctggctcc 6061 agcccctcct caggtgctgc ctcaggctcc tgctcctgca ccagctccag ccatggtgtc 6121 tgcactggct caggcaccag cacccgtgcc tgtgctggct cctggacctc cacaggctgt 6181 ggctccacca gcccctaaac ctacacaggc cggcgagggc acactgtctg aagctctgct 6241 gcagctgcag ttcgacgacg aggatctggg agccctgctg ggaaacagca ccgatcctgc 6301 cgtgttcacc gacctggcca gcgtggacaa cagcgagttc cagcagctgc tgaaccaggg 6361 catccctgtg gcccctcaca ccaccgagcc catgctgatg gaataccccg aggccatcac 6421 ccggctcgtg acaggcgctc agaggcctcc tgatccagct cctgcccctc tgggagcacc 6481 aggcctgcct aatggactgc tgtctggcga cgaggacttc agctctatcg ccgacatgga 6541 cttctccgca ctgctgggta gcggatcggg atctcgggat tccagggaag ggatgttttt 6601 gccgaagcct gaggccggct ccgctattag tgacgtgttt gagggccgcg aggtgtgcca 6661 gccaaaacga atccggccat ttcatcctcc aggaagtcca tgggccaacc gcccactccc 6721 cgccagcctc gcaccaacac caaccggtcc agtacatgag ccagtcgggt cactgacccc 6781 ggcaccagtc cctcagccac tggatccagc gcccgcagtg actcccgagg ccagtcacct 6841 gttggaggat cccgatgaag agacgagcca ggctgtcaaa gcccttcggg agatggccga 6901 tactgtgatt ccccagaagg aagaggctgc aatctgtggc caaatggacc tttcccatcc 6961 gcccccaagg ggccatctgg atgagctgac aaccacactt gagtccatga ccgaggatct 7021 gaacctggac tcacccctga ccccggaatt gaacgagatt ctggatacct tcctgaacga 7081 cgagtgcctc ttgcatgcca tgcatatcag cacaggactg tccatcttcg acacatctct 7141 gttttgagct tacttcttta tcggttctct cttacgactt tttgaatgga acgtttcctt 7201 cttctcaggc gggcctatct ttgggccgaa gctcttttcc ttgtactgta ggacctggtt 7261 gataatgatt cccaaaaaga catccagcat gtcagttact tgcattcgtc agtctataca 7321 aaagcaatgg tttagagaaa ttttgaactt tatacatggt tttatttgtt gcttcacggc 7381 cgtaccttct ggaaatccac ggtaggagtg tcaatttgcg tttttgataa tccttccaag 7441 gttcttctcg aagtagttgt tctataattg cttcacagct accatggaac atcccaagca 7501 ccgctactag aattcgagct cggagtggat cccccgggct gcaggaattc gagctctgta 7561 cagtgaccgg tgactctttc tggcatgcgg agagacggac ggacgcagag agaagggctg 7621 agtaataagc gccactgcgc cagacagctc tggcggctct gaggtgcagt ggatgattat 7681 taatccggga ccggccgccc ctccgccccg aagtggaaag gctggtgtgc ccctcgttga 7741 ccaagaatct attgcatcat cggagaatat ggagcttcat cgaatcaccg gcagtaagcg 7801 aaggagaatg tgaagccagg ggtgtatagc cgtcggcgaa atagcatgcc attaacctag 7861 gtacagaagt ccaattgctt ccgatctggt aaaagattca cgagatagta ccttctccga 7921 agtaggtaga gcgagtaccc ggcgcgtaag ctccctaatt ggcccatccg gcatctgtag 7981 ggcgtccaaa tatcgtgcct ctcctgcttt gcccggtgta tgaaaccgga aaggccgctc 8041 aggagctggc cagcggcgca gaccgggaac acaagctggc agtcgaccca tccggtgctc 8101 tgcactcgac ctgctgaggt ccctcagtcc ctggtaggca gctttgcccc gtctgtccgc 8161 ccggtgtgtc ggcggggttg acaaggtcgt tgcgtcagtc caacatttgt tgccatattt 8221 tcctgctctc cccaccagct gctcttttct tttctctttc ttttcccatc ttcagtatat 8281 tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact 8341 ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc 8401 agcttgacta acagctaccc cgcttgagca gacatcacaa tgaccttgct caatggccac 8461 gcctccctct ccccggccgc tcaacgccgc atcgaacacc atgaagctga tattgtaatt 8521 gtgggtgccg gagtcctcgg ctgtgcactt gcagttgcac tgggaaatca agggcgcagt 8581 gtaatcctgc tggagaagtc gttggaagag cccaaccgta ttgttggtga actactccaa 8641 ccgggaggtg ttcaggcgct cgagcaacta gggctacggg attgtctgga agatatcgat 8701 ggcatcaatg tgaagggata ctgggttact tactttgggg agccggttct cctcgaatac 8761 cccaagtcca gcccaacctc gcctacaccg ttgggacgag ccttccacca tggccgattc 8821 gtgatgaaac tacgggcagc cgctctgtcg tgccccaatg tcacggtggt cgaaaccaag 8881 gttaccggcc tcatcacatc ctcatatact caggaagtcc ttggagtcga gtgtataacc 8941 aaggatgtca aggactgcta cttcggtcaa ctcaccgtgg ccgccgacgg ttataactcc 9001 gttttccgca aggaacacca ccagtataca cccaaacggc gctcaaagtt ccatggcttg 9061 gagctcatcg atgctaaact gcccgctccc aacactggtc atgtgcttct cagcgataat 9121 ccacccgtac ttatgtacca gatcggcacc catgaaaccc gaatccttat cgatatccct 9181 gataacctgc ctgcggcctc ggtcaagaac ggtggtgtca agggttacat gcgaaacaac 9241 atcctgccca agctcccaga gggtgtccag cagtcgttct ccgatgccct agagaagggc 9301 cagctacggt caatgccgaa ctcgttcctg cctgcctcgg ccaacaagac acccggcttg 9361 atgattctgg gcgatgcgct taacatgcgg caccctctca caggtggagg catgactgtg 9421 gcgctcaacg atgtttgtgt catccgcgaa ttgctcagcc cagagcgcgt gcccaacctc 9481 tccaatacag gccttgttct cgagcagctc gcagagttcc actggaaacg aaagaactca 9541 tcttcggtca tcaatatcct cgcacaggct ctctatgcgc tcttcgctgc agacagtacg 9601 cacgctcact cttcaggtta atttgcaata actaactgtt cattcaacag actattacct 9661 caaagctctt cagcgtggct gtttccgcta cttccagatt ggaccagttg gcggccctgt 9721 cgggttgtta gccggtctaa tcaagaaacc cctcgtcctt gtcagccact tcttctccgt 9781 tgccttcctc gccatctggg tccacatatg cgatacccct ctgtccaagc tattcctagc 9841 cccatactac gccgtcatga tattgtacac agcctctgtc gtgattttgc catacatctg 9901 gaccgagatc tggtactgag cttctaataa gtgtcagata gcaatttgca caagaaatca 9961 ataccagcaa ctgtaaataa gcgctgaagt gaccatgcca tgctacgaaa gagcagaaaa 10021 aaacctgccg tagaaccgaa gagatatgac acgcttccat ctctcaaagg aagaatccct 10081 tcagggttgc gtttccagtc tagacacgta taacggcaca agtgtctctc accaaatggg 10141 ttatatctca aatgtgatct aaggatggaa agcccagaat attggctggg ttgatggctg 10201 cttcgagtgc agtctcatgc tgccacaggt gactctggat ggccccatac cactcaaccc 10261 atggtacgag cggtacgctt tacgaattcc catggggagg agccaagagc ggattcctca 10321 gtctcgtacg tctcttgacg accgttgatc tgcttgatct cgtctcccga aaatgaaaat 10381 agactctgct aagctattct tctgcttcgc cggagcctga agggcgtact agggttgcga 10441 ggtccaatgc attaatgcat tgcagatgag ctgtatctgg aagaggtaaa cccgaaacgc 10501 gttttattct tgttgacatg gagctattaa atcagtccca ttcgccatgc cgaagcatgt 10561 tgcccagccg gcgccagcga ggaggctggg accatgccgg ccaaaagcac cgactcggtg 10621 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa 10681 acggagacca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 10741 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 10801 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 10861 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 10921 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 10981 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt 11041 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata 11101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 11161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 11221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgggtctcg tcggtgatgt 11281 ctgctcaagc ggggtagctg ttagtcaagc tgcgatgaag tgggaaagct cgaactgaaa 11341 ggttcaaagg aataagggat gggaaggatg gagtatggat gtagcaaagt acttacttag 11401 gggaaataaa ggttcttgga tgggaagatg aatatactga agatgggaaa agaaagagaa 11461 aagaaaagag cagctggtgg ggagagcagg aaaatatggc aacaaatgtt ggactgacgc 11521 aacgaccttg tcaaccccgc cgacacaccg ggcggacaga cggggcaaag ctgcctacca 11581 gggactgagg gacctcagca ggtcgagtgt agagcaccgg atgggtcgac tgccagcttg 11641 tgttcccggt ctgcgccgct ggccagctcc tgagcggcct ttccggtttc atacaccggg 11701 caaagcagga gaggcacgat atttggacgc cctacagatg ccggatgggc caattaggga 11761 gcttacgccg ctcagagaat tcgcatgcgg agactttaga catacctctc gttgccgact 11821 taaggccgct ccccccctga aagagttgat attgaaggta gtaggtacgg tgcagggtaa 11881 cgcgtgatgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc 11941 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 12001 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 12061 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg 12121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 12181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 12241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 12301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 12361 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 12421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 12481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 12541 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt 12601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 12661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 12721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 12781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 12841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 12901 cgaccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc 12961 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg 13021 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt 13081 tgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca 13141 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 13201 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 13261 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 13321 atccggaatt acgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 13381 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 13441 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 13501 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 13561 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 13621 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 13681 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 13741 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt 13801 aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga 13861 aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta 13921 ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc 13981 ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcctttt 14041 tttctgatca aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 14101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 14161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 14221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 14281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 14341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 14401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 14461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 14521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 14581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 14641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 14701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 14761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 14821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 14881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 14941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 15001 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 15061 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg 15121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc 15181 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgact acatgtatct 15241 gcatgttgca tcgggaaatc ccaccacagg gacagccaag cggccccgcg acttggcagt 15301 gggcaaacta cgcccgattc tggtgccaag aaccgagaag aatgagacag acccacgttg 15361 cactctaacc ggatgctatc gacttacggt ggctgaagat tcaacacgct gcaacgagag 15421 ccaaggtggt ccggacattt tctacgtgcc ggtttacctt ggaacatcgc cgtcgttgag 15481 tgcacgttgc ctactctctc gtggcttggc tgggcccacg agcccgattg actcgacggt 15541 gttacttggg tatctatggc cccgttttct ggcacggtaa tgataagtac ttactagtct 15601 tcgagcgggg gagtgttgct ctgcccgagc atcaacgatt ggcctgatcg caccgtctgc 15661 aaatgccacg gtgcggaccg actgaaatct cagaccacca aagaccctcc gacttcgaga 15721 tacggttact aattttacac tggctccagc ggccccatcc agtaagcatc tgggctgcaa 15781 gcgtataatg tctccaggtt gtctcagcat aaacaccccg cccccgctca ggcacacagg 15841 aagagagctc aggtcgtttc cattgcgtcc atactcttca ctcattgtca tctgcaggag 15901 aacttcccct gtccctttgc caagccctct cttcgtcgtt gtccacgcct tcaagttttc 15961 accattattt ttctagacac catggccaag ttgaccagtg ccgttccggt gctcaccgcg 16021 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc 16081 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc 16141 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag 16201 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc 16261 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc 16321 aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt 16381 ccgacgcggc ccgacgggtc cgaggcctcg gagatccgtc ccccttttcc tttgtcgata 16441 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg 16501 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt 16561 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt 16621 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt 16681 taatttgcaa gcttgatagc ttattttttg tatactgttt tgtgatagca cgaagttttt 16741 ccacggtatc ttgttaaaaa tatatatttg tggcgggctt acctacatca aattaataag 16801 agactaatta taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct 16861 tttgacgtat aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag 16921 agactagtta gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa 16981 cagaacctag aaggggacta gatatccggg tagggaaaca aaaaaaaaaa acaagacagc 17041 cacatattag ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga 17101 taccacagtc tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc 17161 aggttagttg caactaatac tagttagtat ctagtctcct ataactctga agctagaata 17221 acttactact attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt 17281 cagagactag ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca 17341 tgtctgccat caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc 17401 tcattttcct tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa 17461 atgtcttacc actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt 17521 agagtctagt tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac 17581 tctccagggc ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg 17641 tctttgtctg agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat 17701 gagaaaaagc gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag 17761 aatctagtcc caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt 17821 gcagggtatg gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat 17881 ctgcataaag agtggctaga ggttataaat tagcactgat cctaggtacg gggctgaatg 17941 taacttgcct ttcctttctc atcgcgcggc aagacaggct tgctcaaatt cctaccagtc 18001 acaggggtat gcacggcgta cggaccactt gaactagtca cagattagtt agcaactagt 18061 ctgcattgaa tggctgtact tacgggccct cgccattgtc ctgatcattt ccagcttcac 18121 cctcgttgct gcaaagtagt tagtgactag tcaaggacta gttgaaatgg gagaagaaac 18181 tcacgaattc tcgacaccct tagtattgtg gtccttggac ttggtgctgc tatatattag 18241 ctaatacact agttagactc acagaaactt acgcagctcg cttgcgcttc ttggtaggag 18301 tcggggttgg gagaacagtg ccttcaaaca agccttcata ccatgctact tgactagtca 18361 gggactagtc accaagtaat ctagatagga cttgcctttg gcctccatca gttccttcat 18421 agtgggaggt ccattgtgca atgtaaactc catgccgtgg gagttcttgt ccttcaagtg 18481 cttgaccaat atgtttctgt tggcagaggg aacctgtcaa ctagttaata actagtcaga 18541 aactagtata gcagtagact cactgtacgc ttgaggcatc ccttcactcg gcagtagact 18601 tcatatggat ggatatcagg cacgccattg tcgtcctgtg gactagtcag taactaggct 18661 taaagctagt cgggtcggct tactatcttg aaatccggca gcgtaagctc cccgtcctta 18721 actgcctcga gatagtgaca gtactctggg gactttcgga gatcgttatc gcgaatgctc 18781 ggcatactaa tcgttgacta gtcttggact agtcccgagc aaaaaggatt ggaggaggag 18841 gaggaaggtg agagtgagac aaagagcgaa ataagagctt caaaggctat ctctaagcag 18901 tatgaaggtt aagtatctag ttcttgacta gatttaaaag agatttcgac tagttatgta 18961 cctggagttt ggatatagga atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa 19021 agtcggtcaa gaggtaactc taagtcggcc attccttttt gggaggcgct aaccataaac 19081 ggcatggtcg acttagagtt agctcaggga atttagggag ttatctgcga ccaccgagga 19141 acggcggaat gccaaagaat cccgatggag ctctagctgg cggttgacaa ccccaccttt 19201 tggcgtttct gcggcgttgc aggcgggact ggatacttcg tagaaccaga aaggcaaggc 19261 agaacgcgct cagcaagagt gttggaagtg atagcatgat gtgccttgtt aactaggtca 19321 aaatctgcag tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca aacatacacg 19381 attgggagag ggcctaggta taagagtttt tgagtagaac gcatgtgagc ccagccatct 19441 cgaggagatt aaacacgggc cggcatttga tggctatgtt agtaccccaa tggaaagcct 19501 gagagtccag tggtcgcaga taactcccta aattccctga gctaactcta agtcgaccat 19561 gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac ctcttgaccg 19621 actttttctt tcctccccct tacatttcgt taccacaaca cattcctata tccaaactcc 19681 aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata cttaaccttc 19741 atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac tctcaccttc 19801 ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa cgattagtat 19861 gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact atctcgaggc 19921 agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac ccgactagct 19981 ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata tccatccata 20041 tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac tgctatacta 20101 gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa catattggtc 20161 aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa tggacctccc 20221 actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt ggtgactagt 20281 ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt ctcccaaccc 20341 cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta actagtgtat 20401 tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt cgagaattcg 20461 tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt tgcagcaacg 20521 agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag ccattcaatg 20581 cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc 20641 tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag 20701 ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc actctttatg 20761 cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc 20821 tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag 20881 attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt 20941 ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa 21001 agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg 21061 agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac 21121 tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga 21181 catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa 21241 tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag 21301 acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct 21361 ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta 21421 agttattcta gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa 21481 cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg 21541 gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat 21601 gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt 21661 ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag 21721 tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc 21781 aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag 21841 tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg 21901 tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctatc //