pLM-AMA18.0 dCas9-VPR

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pLM-AMA18.0 dCas9-VPR
出品公司: Addgene
目录编号: 138945
存储实验室: Arnold Driessen

载体骨架基本信息
载体名称: pDSM-JAK-109
载体出品公司: DSM
空载体大小: 10170 bp
完整质粒大小: --
载体修饰: pGpda:DsRed SKL已删除
载体类型: CRISPR,合成生物学;AMA1自主复制真菌质粒
筛选标记: 真菌选择:特比萘芬(ergA)/静脉霉素/博莱霉素(ble)
载体抗性: 氯霉素
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: --

插入基因信息
插入基因名称: p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
物种来源: 合成的真菌表达,红色青霉
插入大小: 21950 bp
基因突变: --
GenBank编号 --
启动子: p40S(AN0465)巢状曲霉
标签/融合蛋白: NLS
克隆方法: Gibson克隆
5’克隆位点: --
3’克隆位点: --
5’测序引物 --
3’测序引物 --

文献引用方法
材料和方法部分: pLM-AMA18.0 dCas9-VPR was a gift from Arnold Driessen (Addgene plasmid # 138945 ; http://n2t.net/addgene:138945 ; RRID:Addgene_138945)
参考文献部分: CRISPR-based transcriptional activation tool for silent genes in filamentous fungi. Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen AJM. Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3. 10.1038/s41598-020-80864-3 PubMed 33441979

载体图谱质粒图谱和多克隆位点信息

pLM-AMA18.0 dCas9-VPR质粒图谱

载体简介

载体序列

LOCUS       pLM-AMA18.0 dCas9-VPR             21949 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta 
            (VPR); ribozymes based sgRNA transcription unit; Terbinafine and 
            Phleomycin selection markers.
ACCESSION   .
VERSION     .
KEYWORDS    pLM-AMA18.0 dCas9-VPR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21949)
  AUTHORS   Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen 
            AJM
  TITLE     CRISPR-based transcriptional activation tool for silent genes in 
            filamentous fungi.
  JOURNAL   Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.
  PUBMED    33441979
REFERENCE   2  (bases 1 to 21949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..21949
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1415..5518
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5531..5551
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5576..5725
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5750..5770
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6200..6556
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6575..7144
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     intron          8316..8431
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     misc_RNA        complement(10607..10682)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(10776..11099)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     10781..10800
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11009..11025
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11026..11082
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11095..11111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11119..11135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11143..11173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11188..11209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     intron          11281..11396
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(11918..11937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11919..11937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11947..11963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      12105..12560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12192..12211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12402..12423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12586..12690
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13010..13112
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             13113..13772
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13179..13198)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      14161..14749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14650..14669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14903..14920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15037..15058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        15073..15103
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15111..15127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15135..15151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15170..15190
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15172..15190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             15982..16356
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      16441..16688
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(16457..16475)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      16698..21946
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 gaattcctgc agccccagat catcctgtct tcagtcttaa cgctgcaaga attcaagctt
       61 ggaggagtta tagacggtcc ggcataggta aggagagatt agatatttcg acttcccgat
      121 tccttagtct ccttagtctg gaaactgaga aaggcctgaa ctcgttcaat ctattagctc
      181 taatcacgta gtctcaacta gaatcctgaa caggcgccgg aaccagtcca aatgcgtgga
      241 gtacagcaag tccagtcaaa tcacgattag aatggccata tataagcaaa aaacccttgg
      301 acaatacgtc cgatcgacac caaagcaatc tggtacatca cccttcttat atacagggta
      361 gccggaattg tattgactct atacgaagcc gcaggtttac cagcacagag gtgcgatagc
      421 tacacgaatg tagagtgaag gcattacaac cgattgctaa gtgtcataga ggctaagttt
      481 cacggctcaa ggagtctgtg gaggatacct gatataagaa ttgagagtta tactccggag
      541 gttatgttag tacctcaaaa atgtgaagta caacgaaact cgaacttcga atgacaatag
      601 cttggctggc tgttagtcaa ctgaggtaca acgtcatctt gcaattagtt atgttctttg
      661 ctagatagtg agctgctgta aacttgtagt tcttagaaac atgtctggag tctagagtcg
      721 agacttgtga ttggtgtaaa ttagtggggg tccggagcgg aataacaatc gaggtcagcc
      781 tcctgggtct gtcagtaagc taaccacgaa aggacaagcg tagacgaggt cacggggtac
      841 tggcctagcc ggcttcggct agcgcccacg aaatagggca acgtcgaagc gagagacaca
      901 cacggattcc acgatcgccg acgacaagca ctcaactcca cgacaaccca cgacaaccct
      961 tgccgctgcg ttcttggtga agggctgcag gattagaggt gagtatttta ttttctggct
     1021 attgtgatct ttttcccccc cggtttccgc tggttctatc ttgtcgagcg agtcggtgcg
     1081 ccttcgatcg gtctgtcaaa tttccatccg tccactcgtt cacccggtat cgatacaaga
     1141 gggaatggag cattacgaca ggggagcaga ggcagaactc tcgatgttgc tacttttttt
     1201 gcttggaaca acttccttga aagattcttt tctgaatgcg gctgggaaac tgcgaattgg
     1261 atatccagcg aaaggttacc gacagactca cggccatcga taccactcag cgggatagaa
     1321 ttgaaggcat tcggacatta gatgatcagc ttcgcttgtt tcgactcgtg ctaatcaatt
     1381 atccctggac agtcccccac atagacagca aacaatggac aagaagtatt ctatcggact
     1441 ggccatcggg actaatagcg tcgggtgggc cgtgatcact gacgagtaca aggtgccctc
     1501 taagaagttc aaggtgctcg ggaacaccga ccggcattcc atcaagaaaa atctgatcgg
     1561 agctctcctc tttgattcag gggaaaccgc tgaagcaacc cgcctcaagc ggactgctag
     1621 acggcggtac accaggagga agaaccggat ttgttacctt caagagatat tctccaacga
     1681 aatggcaaag gtcgacgaca gcttcttcca taggctggaa gaatcattcc tcgtggaaga
     1741 ggataagaag catgaacggc atcccatctt cggtaatatc gtcgacgagg tggcctatca
     1801 cgagaaatac ccaaccatct accatcttcg caaaaagctg gtggactcaa ccgacaaggc
     1861 agacctccgg cttatctacc tggccctggc ccacatgatc aagttcagag gccacttcct
     1921 gatcgagggc gacctcaatc ctgacaatag cgatgtggat aaactgttca tccagctggt
     1981 gcagacttac aaccagctct ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa
     2041 ggccattctg tcagcccggc tgtcaaagag ccgcagactt gagaatctta tcgctcagct
     2101 gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt gctctttcac ttgggctgac
     2161 tcccaatttc aagtctaatt tcgacctggc agaggatgcc aagctgcaac tgtccaagga
     2221 cacctatgat gacgatctcg acaacctcct ggcccagatc ggtgaccaat acgccgacct
     2281 tttccttgct gctaagaatc tttctgacgc catcctgctg tctgacattc tccgcgtgaa
     2341 cactgaaatc accaaggccc ctctttcagc ttcaatgatt aagcggtatg atgagcacca
     2401 ccaggacctg accctgctta aggcactcgt ccggcagcag cttccggaga agtacaagga
     2461 aatcttcttt gaccagtcaa agaatggata cgccggctac atcgacggag gtgcctccca
     2521 agaggaattt tataagttta tcaaacctat ccttgagaag atggacggca ccgaagagct
     2581 cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag cgcactttcg acaatgggag
     2641 cattccccac cagatccatc ttggggagct tcacgccatc cttcggcgcc aagaggactt
     2701 ctaccccttt cttaaggaca acagggagaa gattgagaaa attctcactt tccgcatccc
     2761 ctactacgtg ggacccctcg ccagaggaaa tagccggttt gcttggatga ccagaaagtc
     2821 agaagaaact atcactccct ggaacttcga agaggtggtg gacaagggag ccagcgctca
     2881 gtcattcatc gaacggatga ctaacttcga taagaacctc cccaatgaga aggtcctgcc
     2941 gaaacattcc ctgctctacg agtactttac cgtgtacaac gagctgacca aggtgaaata
     3001 tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt
     3061 ggaccttctg ttcaagacca atagaaaggt gaccgtgaag cagctgaagg aggactattt
     3121 caagaaaatt gaatgcttcg actctgtgga gattagcggg gtcgaagatc ggttcaacgc
     3181 aagcctgggt acttaccatg atctgcttaa gatcatcaag gacaaggatt ttctggacaa
     3241 tgaggagaac gaggacatcc ttgaggacat tgtcctgact ctcactctgt tcgaggaccg
     3301 ggaaatgatc gaggagaggc ttaagaccta cgcccatctg ttcgacgata aagtgatgaa
     3361 gcaacttaaa cggagaagat ataccggatg gggacgcctt agccgcaaac tcatcaacgg
     3421 aatccgggac aaacagagcg gaaagaccat tcttgatttc cttaagagcg acggattcgc
     3481 taatcgcaac ttcatgcaac ttatccatga tgattccctg acctttaagg aggacatcca
     3541 gaaggcccaa gtgtctggac aaggtgactc actgcacgag catatcgcaa atctggctgg
     3601 ttcacccgct attaagaagg gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa
     3661 ggtgatgggt cgccataaac cagagaacat tgtcatcgag atggccaggg aaaaccagac
     3721 tacccagaag ggacagaaga acagcaggga gcggatgaaa agaattgagg aagggattaa
     3781 ggagctcggg tcacagatcc ttaaagagca cccggtggaa aacacccagc ttcagaatga
     3841 gaagctctat ctgtactacc ttcaaaatgg acgcgatatg tatgtggacc aagagcttga
     3901 tatcaacagg ctctcagact acgacgtggc tgccatcgtc cctcagagct tcctcaaaga
     3961 cgactcaatt gacaataagg tgctgactcg ctcagacaag gcccggggaa agtcagataa
     4021 cgtgccctca gaggaagtcg tgaaaaagat gaagaactat tggcgccagc ttctgaacgc
     4081 aaagctgatc actcagcgga agttcgacaa tctcactaag gctgagaggg gcggactgag
     4141 cgaactggac aaagcaggat tcattaaacg gcaacttgtg gagactcggc agattactaa
     4201 acatgtcgcc caaatccttg actcacgcat gaataccaag tacgacgaaa acgacaaact
     4261 tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga
     4321 ctttcaattc tacaaagtgc gggagatcaa taactatcat catgctcatg acgcatatct
     4381 gaatgccgtg gtgggaaccg ccctgatcaa gaagtaccca aagctggaaa gcgagttcgt
     4441 gtacggagac tacaaggtct acgacgtgcg caagatgatt gccaaatctg agcaggagat
     4501 cggaaaggcc accgcaaagt acttcttcta cagcaacatc atgaatttct tcaagaccga
     4561 aatcaccctt gcaaacggtg agatccggaa gaggccgctc atcgagacta atggggagac
     4621 tggcgaaatc gtgtgggaca agggcagaga tttcgctacc gtgcgcaaag tgctttctat
     4681 gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc
     4741 aatcctcccc aagcgcaact ccgacaagct cattgcaagg aagaaggatt gggaccctaa
     4801 gaagtacggc ggattcgatt caccaactgt ggcttattct gtcctggtcg tggctaaggt
     4861 ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa ctgctgggta tcaccattat
     4921 ggagcgcagc tccttcgaga agaacccaat tgactttctc gaagccaaag gttacaagga
     4981 agtcaagaag gaccttatca tcaagctccc aaagtatagc ctgttcgaac tggagaatgg
     5041 gcggaagcgg atgctcgcct ccgctggcga acttcagaag ggtaatgagc tggctctccc
     5101 ctccaagtac gtgaatttcc tctaccttgc aagccattac gagaagctga aggggagccc
     5161 cgaggacaac gagcaaaagc aactgtttgt ggagcagcat aagcattatc tggacgagat
     5221 cattgagcag atttccgagt tttctaaacg cgtcattctc gctgatgcca acctcgataa
     5281 agtccttagc gcatacaata agcacagaga caaaccaatt cgggagcagg ctgagaatat
     5341 catccacctg ttcaccctca ccaatcttgg tgcccctgcc gcattcaagt acttcgacac
     5401 caccatcgac cggaaacgct atacctccac caaagaagtg ctggacgcca ccctcatcca
     5461 ccagagcatc accggacttt acgaaactcg gattgacctc tcacagctcg gaggggatag
     5521 cagggctgac cccaagaaga agaggaaggt ggaggccagc ggttccggac gggctgacgc
     5581 attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga
     5641 catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct
     5701 tgatgatttc gacctggaca tgctgattaa ctctagaagt tccggatctc cgaaaaagaa
     5761 acgcaaagtt ggtagccagt acctgcccga caccgacgac cggcaccgga tcgaggaaaa
     5821 gcggaagcgg acctacgaga cattcaagag catcatgaag aagtccccct tcagcggccc
     5881 caccgaccct agacctccac ctagaagaat cgccgtgccc agcagatcca gcgccagcgt
     5941 gccaaaacct gccccccagc cttacccctt caccagcagc ctgagcacca tcaactacga
     6001 cgagttccct accatggtgt tccccagcgg ccagatctct caggcctctg ctctggctcc
     6061 agcccctcct caggtgctgc ctcaggctcc tgctcctgca ccagctccag ccatggtgtc
     6121 tgcactggct caggcaccag cacccgtgcc tgtgctggct cctggacctc cacaggctgt
     6181 ggctccacca gcccctaaac ctacacaggc cggcgagggc acactgtctg aagctctgct
     6241 gcagctgcag ttcgacgacg aggatctggg agccctgctg ggaaacagca ccgatcctgc
     6301 cgtgttcacc gacctggcca gcgtggacaa cagcgagttc cagcagctgc tgaaccaggg
     6361 catccctgtg gcccctcaca ccaccgagcc catgctgatg gaataccccg aggccatcac
     6421 ccggctcgtg acaggcgctc agaggcctcc tgatccagct cctgcccctc tgggagcacc
     6481 aggcctgcct aatggactgc tgtctggcga cgaggacttc agctctatcg ccgacatgga
     6541 cttctccgca ctgctgggta gcggatcggg atctcgggat tccagggaag ggatgttttt
     6601 gccgaagcct gaggccggct ccgctattag tgacgtgttt gagggccgcg aggtgtgcca
     6661 gccaaaacga atccggccat ttcatcctcc aggaagtcca tgggccaacc gcccactccc
     6721 cgccagcctc gcaccaacac caaccggtcc agtacatgag ccagtcgggt cactgacccc
     6781 ggcaccagtc cctcagccac tggatccagc gcccgcagtg actcccgagg ccagtcacct
     6841 gttggaggat cccgatgaag agacgagcca ggctgtcaaa gcccttcggg agatggccga
     6901 tactgtgatt ccccagaagg aagaggctgc aatctgtggc caaatggacc tttcccatcc
     6961 gcccccaagg ggccatctgg atgagctgac aaccacactt gagtccatga ccgaggatct
     7021 gaacctggac tcacccctga ccccggaatt gaacgagatt ctggatacct tcctgaacga
     7081 cgagtgcctc ttgcatgcca tgcatatcag cacaggactg tccatcttcg acacatctct
     7141 gttttgagct tacttcttta tcggttctct cttacgactt tttgaatgga acgtttcctt
     7201 cttctcaggc gggcctatct ttgggccgaa gctcttttcc ttgtactgta ggacctggtt
     7261 gataatgatt cccaaaaaga catccagcat gtcagttact tgcattcgtc agtctataca
     7321 aaagcaatgg tttagagaaa ttttgaactt tatacatggt tttatttgtt gcttcacggc
     7381 cgtaccttct ggaaatccac ggtaggagtg tcaatttgcg tttttgataa tccttccaag
     7441 gttcttctcg aagtagttgt tctataattg cttcacagct accatggaac atcccaagca
     7501 ccgctactag aattcgagct cggagtggat cccccgggct gcaggaattc gagctctgta
     7561 cagtgaccgg tgactctttc tggcatgcgg agagacggac ggacgcagag agaagggctg
     7621 agtaataagc gccactgcgc cagacagctc tggcggctct gaggtgcagt ggatgattat
     7681 taatccggga ccggccgccc ctccgccccg aagtggaaag gctggtgtgc ccctcgttga
     7741 ccaagaatct attgcatcat cggagaatat ggagcttcat cgaatcaccg gcagtaagcg
     7801 aaggagaatg tgaagccagg ggtgtatagc cgtcggcgaa atagcatgcc attaacctag
     7861 gtacagaagt ccaattgctt ccgatctggt aaaagattca cgagatagta ccttctccga
     7921 agtaggtaga gcgagtaccc ggcgcgtaag ctccctaatt ggcccatccg gcatctgtag
     7981 ggcgtccaaa tatcgtgcct ctcctgcttt gcccggtgta tgaaaccgga aaggccgctc
     8041 aggagctggc cagcggcgca gaccgggaac acaagctggc agtcgaccca tccggtgctc
     8101 tgcactcgac ctgctgaggt ccctcagtcc ctggtaggca gctttgcccc gtctgtccgc
     8161 ccggtgtgtc ggcggggttg acaaggtcgt tgcgtcagtc caacatttgt tgccatattt
     8221 tcctgctctc cccaccagct gctcttttct tttctctttc ttttcccatc ttcagtatat
     8281 tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta catccatact
     8341 ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc acttcatcgc
     8401 agcttgacta acagctaccc cgcttgagca gacatcacaa tgaccttgct caatggccac
     8461 gcctccctct ccccggccgc tcaacgccgc atcgaacacc atgaagctga tattgtaatt
     8521 gtgggtgccg gagtcctcgg ctgtgcactt gcagttgcac tgggaaatca agggcgcagt
     8581 gtaatcctgc tggagaagtc gttggaagag cccaaccgta ttgttggtga actactccaa
     8641 ccgggaggtg ttcaggcgct cgagcaacta gggctacggg attgtctgga agatatcgat
     8701 ggcatcaatg tgaagggata ctgggttact tactttgggg agccggttct cctcgaatac
     8761 cccaagtcca gcccaacctc gcctacaccg ttgggacgag ccttccacca tggccgattc
     8821 gtgatgaaac tacgggcagc cgctctgtcg tgccccaatg tcacggtggt cgaaaccaag
     8881 gttaccggcc tcatcacatc ctcatatact caggaagtcc ttggagtcga gtgtataacc
     8941 aaggatgtca aggactgcta cttcggtcaa ctcaccgtgg ccgccgacgg ttataactcc
     9001 gttttccgca aggaacacca ccagtataca cccaaacggc gctcaaagtt ccatggcttg
     9061 gagctcatcg atgctaaact gcccgctccc aacactggtc atgtgcttct cagcgataat
     9121 ccacccgtac ttatgtacca gatcggcacc catgaaaccc gaatccttat cgatatccct
     9181 gataacctgc ctgcggcctc ggtcaagaac ggtggtgtca agggttacat gcgaaacaac
     9241 atcctgccca agctcccaga gggtgtccag cagtcgttct ccgatgccct agagaagggc
     9301 cagctacggt caatgccgaa ctcgttcctg cctgcctcgg ccaacaagac acccggcttg
     9361 atgattctgg gcgatgcgct taacatgcgg caccctctca caggtggagg catgactgtg
     9421 gcgctcaacg atgtttgtgt catccgcgaa ttgctcagcc cagagcgcgt gcccaacctc
     9481 tccaatacag gccttgttct cgagcagctc gcagagttcc actggaaacg aaagaactca
     9541 tcttcggtca tcaatatcct cgcacaggct ctctatgcgc tcttcgctgc agacagtacg
     9601 cacgctcact cttcaggtta atttgcaata actaactgtt cattcaacag actattacct
     9661 caaagctctt cagcgtggct gtttccgcta cttccagatt ggaccagttg gcggccctgt
     9721 cgggttgtta gccggtctaa tcaagaaacc cctcgtcctt gtcagccact tcttctccgt
     9781 tgccttcctc gccatctggg tccacatatg cgatacccct ctgtccaagc tattcctagc
     9841 cccatactac gccgtcatga tattgtacac agcctctgtc gtgattttgc catacatctg
     9901 gaccgagatc tggtactgag cttctaataa gtgtcagata gcaatttgca caagaaatca
     9961 ataccagcaa ctgtaaataa gcgctgaagt gaccatgcca tgctacgaaa gagcagaaaa
    10021 aaacctgccg tagaaccgaa gagatatgac acgcttccat ctctcaaagg aagaatccct
    10081 tcagggttgc gtttccagtc tagacacgta taacggcaca agtgtctctc accaaatggg
    10141 ttatatctca aatgtgatct aaggatggaa agcccagaat attggctggg ttgatggctg
    10201 cttcgagtgc agtctcatgc tgccacaggt gactctggat ggccccatac cactcaaccc
    10261 atggtacgag cggtacgctt tacgaattcc catggggagg agccaagagc ggattcctca
    10321 gtctcgtacg tctcttgacg accgttgatc tgcttgatct cgtctcccga aaatgaaaat
    10381 agactctgct aagctattct tctgcttcgc cggagcctga agggcgtact agggttgcga
    10441 ggtccaatgc attaatgcat tgcagatgag ctgtatctgg aagaggtaaa cccgaaacgc
    10501 gttttattct tgttgacatg gagctattaa atcagtccca ttcgccatgc cgaagcatgt
    10561 tgcccagccg gcgccagcga ggaggctggg accatgccgg ccaaaagcac cgactcggtg
    10621 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
    10681 acggagacca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    10741 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    10801 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    10861 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    10921 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    10981 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
    11041 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
    11101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgggtctcg tcggtgatgt
    11281 ctgctcaagc ggggtagctg ttagtcaagc tgcgatgaag tgggaaagct cgaactgaaa
    11341 ggttcaaagg aataagggat gggaaggatg gagtatggat gtagcaaagt acttacttag
    11401 gggaaataaa ggttcttgga tgggaagatg aatatactga agatgggaaa agaaagagaa
    11461 aagaaaagag cagctggtgg ggagagcagg aaaatatggc aacaaatgtt ggactgacgc
    11521 aacgaccttg tcaaccccgc cgacacaccg ggcggacaga cggggcaaag ctgcctacca
    11581 gggactgagg gacctcagca ggtcgagtgt agagcaccgg atgggtcgac tgccagcttg
    11641 tgttcccggt ctgcgccgct ggccagctcc tgagcggcct ttccggtttc atacaccggg
    11701 caaagcagga gaggcacgat atttggacgc cctacagatg ccggatgggc caattaggga
    11761 gcttacgccg ctcagagaat tcgcatgcgg agactttaga catacctctc gttgccgact
    11821 taaggccgct ccccccctga aagagttgat attgaaggta gtaggtacgg tgcagggtaa
    11881 cgcgtgatgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc
    11941 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    12001 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    12061 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
    12121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    12181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    12241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    12301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    12361 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    12421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    12481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    12541 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    12601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    12661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
    12721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
    12781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
    12841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
    12901 cgaccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc
    12961 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg
    13021 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
    13081 tgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
    13141 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
    13201 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
    13261 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
    13321 atccggaatt acgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
    13381 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
    13441 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
    13501 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
    13561 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
    13621 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
    13681 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
    13741 agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt
    13801 aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga
    13861 aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta
    13921 ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc
    13981 ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcctttt
    14041 tttctgatca aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    14101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    14161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    14221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    14281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    14341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    14401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    14461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    14521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    14581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    14641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    14701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    14761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    14821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    14881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    14941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    15001 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    15061 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
    15121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    15181 tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaggtcgact acatgtatct
    15241 gcatgttgca tcgggaaatc ccaccacagg gacagccaag cggccccgcg acttggcagt
    15301 gggcaaacta cgcccgattc tggtgccaag aaccgagaag aatgagacag acccacgttg
    15361 cactctaacc ggatgctatc gacttacggt ggctgaagat tcaacacgct gcaacgagag
    15421 ccaaggtggt ccggacattt tctacgtgcc ggtttacctt ggaacatcgc cgtcgttgag
    15481 tgcacgttgc ctactctctc gtggcttggc tgggcccacg agcccgattg actcgacggt
    15541 gttacttggg tatctatggc cccgttttct ggcacggtaa tgataagtac ttactagtct
    15601 tcgagcgggg gagtgttgct ctgcccgagc atcaacgatt ggcctgatcg caccgtctgc
    15661 aaatgccacg gtgcggaccg actgaaatct cagaccacca aagaccctcc gacttcgaga
    15721 tacggttact aattttacac tggctccagc ggccccatcc agtaagcatc tgggctgcaa
    15781 gcgtataatg tctccaggtt gtctcagcat aaacaccccg cccccgctca ggcacacagg
    15841 aagagagctc aggtcgtttc cattgcgtcc atactcttca ctcattgtca tctgcaggag
    15901 aacttcccct gtccctttgc caagccctct cttcgtcgtt gtccacgcct tcaagttttc
    15961 accattattt ttctagacac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
    16021 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
    16081 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
    16141 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
    16201 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
    16261 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
    16321 aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa caccgccggt
    16381 ccgacgcggc ccgacgggtc cgaggcctcg gagatccgtc ccccttttcc tttgtcgata
    16441 tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg
    16501 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt
    16561 tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt
    16621 acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt
    16681 taatttgcaa gcttgatagc ttattttttg tatactgttt tgtgatagca cgaagttttt
    16741 ccacggtatc ttgttaaaaa tatatatttg tggcgggctt acctacatca aattaataag
    16801 agactaatta taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct
    16861 tttgacgtat aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag
    16921 agactagtta gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa
    16981 cagaacctag aaggggacta gatatccggg tagggaaaca aaaaaaaaaa acaagacagc
    17041 cacatattag ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga
    17101 taccacagtc tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc
    17161 aggttagttg caactaatac tagttagtat ctagtctcct ataactctga agctagaata
    17221 acttactact attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt
    17281 cagagactag ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca
    17341 tgtctgccat caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc
    17401 tcattttcct tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa
    17461 atgtcttacc actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt
    17521 agagtctagt tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac
    17581 tctccagggc ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg
    17641 tctttgtctg agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat
    17701 gagaaaaagc gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag
    17761 aatctagtcc caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt
    17821 gcagggtatg gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat
    17881 ctgcataaag agtggctaga ggttataaat tagcactgat cctaggtacg gggctgaatg
    17941 taacttgcct ttcctttctc atcgcgcggc aagacaggct tgctcaaatt cctaccagtc
    18001 acaggggtat gcacggcgta cggaccactt gaactagtca cagattagtt agcaactagt
    18061 ctgcattgaa tggctgtact tacgggccct cgccattgtc ctgatcattt ccagcttcac
    18121 cctcgttgct gcaaagtagt tagtgactag tcaaggacta gttgaaatgg gagaagaaac
    18181 tcacgaattc tcgacaccct tagtattgtg gtccttggac ttggtgctgc tatatattag
    18241 ctaatacact agttagactc acagaaactt acgcagctcg cttgcgcttc ttggtaggag
    18301 tcggggttgg gagaacagtg ccttcaaaca agccttcata ccatgctact tgactagtca
    18361 gggactagtc accaagtaat ctagatagga cttgcctttg gcctccatca gttccttcat
    18421 agtgggaggt ccattgtgca atgtaaactc catgccgtgg gagttcttgt ccttcaagtg
    18481 cttgaccaat atgtttctgt tggcagaggg aacctgtcaa ctagttaata actagtcaga
    18541 aactagtata gcagtagact cactgtacgc ttgaggcatc ccttcactcg gcagtagact
    18601 tcatatggat ggatatcagg cacgccattg tcgtcctgtg gactagtcag taactaggct
    18661 taaagctagt cgggtcggct tactatcttg aaatccggca gcgtaagctc cccgtcctta
    18721 actgcctcga gatagtgaca gtactctggg gactttcgga gatcgttatc gcgaatgctc
    18781 ggcatactaa tcgttgacta gtcttggact agtcccgagc aaaaaggatt ggaggaggag
    18841 gaggaaggtg agagtgagac aaagagcgaa ataagagctt caaaggctat ctctaagcag
    18901 tatgaaggtt aagtatctag ttcttgacta gatttaaaag agatttcgac tagttatgta
    18961 cctggagttt ggatatagga atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa
    19021 agtcggtcaa gaggtaactc taagtcggcc attccttttt gggaggcgct aaccataaac
    19081 ggcatggtcg acttagagtt agctcaggga atttagggag ttatctgcga ccaccgagga
    19141 acggcggaat gccaaagaat cccgatggag ctctagctgg cggttgacaa ccccaccttt
    19201 tggcgtttct gcggcgttgc aggcgggact ggatacttcg tagaaccaga aaggcaaggc
    19261 agaacgcgct cagcaagagt gttggaagtg atagcatgat gtgccttgtt aactaggtca
    19321 aaatctgcag tatgcttgat gttatccaaa gtgtgagaga ggaaggtcca aacatacacg
    19381 attgggagag ggcctaggta taagagtttt tgagtagaac gcatgtgagc ccagccatct
    19441 cgaggagatt aaacacgggc cggcatttga tggctatgtt agtaccccaa tggaaagcct
    19501 gagagtccag tggtcgcaga taactcccta aattccctga gctaactcta agtcgaccat
    19561 gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac ctcttgaccg
    19621 actttttctt tcctccccct tacatttcgt taccacaaca cattcctata tccaaactcc
    19681 aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata cttaaccttc
    19741 atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac tctcaccttc
    19801 ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa cgattagtat
    19861 gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact atctcgaggc
    19921 agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac ccgactagct
    19981 ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata tccatccata
    20041 tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac tgctatacta
    20101 gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa catattggtc
    20161 aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa tggacctccc
    20221 actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt ggtgactagt
    20281 ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt ctcccaaccc
    20341 cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta actagtgtat
    20401 tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt cgagaattcg
    20461 tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt tgcagcaacg
    20521 agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag ccattcaatg
    20581 cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc
    20641 tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag
    20701 ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc actctttatg
    20761 cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc
    20821 tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag
    20881 attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt
    20941 ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa
    21001 agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg
    21061 agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac
    21121 tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga
    21181 catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa
    21241 tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag
    21301 acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct
    21361 ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta
    21421 agttattcta gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa
    21481 cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg
    21541 gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat
    21601 gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt
    21661 ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag
    21721 tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc
    21781 aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag
    21841 tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg
    21901 tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctatc
//

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